![]() May be useful for repeated measurements when quick turnaround time is preferred.Results may be expressed in a variety of units or only qualitatively.Results may be reported in hours or days.May detect only a subset of Legionella species or serogroups.Should be verified by the laboratory (e.g., per ISO 17025).Should be validated against a standard method by a third party (e.g., ISO/IEC 13843).May be preferred for evaluating whether remediation was successful.Results typically reported in 2–48 hours.Results typically reported in genomic units (GU) which is not directly equivalent to CFU.May not differentiate between live and dead bacteria.May be preferred for evaluating growth trends.Is subject to skill, experience, and procedural rigor of the laboratory.Results typically reported in 7–14 days.Yields isolate for additional characterization.Results typically reported in colony forming units (CFU) per volume with limit of detection ~10 CFU/mL.Sample volumes processed, plate types, resuspension procedures, and result reporting vary by lab even when using standard operating procedures such as CDC methods or ISO 11731 from the International Organization of Standardization. ![]() Note: Test method may vary by the type of water system and the reason for testing. Test method may vary by the type of water system and the reason for testing. ![]() Capacity to perform additional Legionella characterization as needed by the submitter.Capability of retaining Legionella isolates from samples for additional characterization.Accreditation by a regional, national, or international accrediting body to a recognized standard for routine Legionella test methods, such as ISO/IEC 17025.For best results, follow instructions from the manufacturer or testing laboratory closely.Ĭonsiderations when working with laboratories testing for Legionella: Regardless of the test method, be sure that you understand the performance characteristics of the test such as sensitivity, specificity, and limitations. Some test methods may be performed onsite by the user or a qualified technician, while other methods may require contracting with a commercial laboratory. Laboratory Considerations and Test Methods Reference CDC Sampling Procedure and Potential Sampling Sites for Investigations for step-by-step instructions on selecting sites and collecting samples.Larger sample volumes and other sample types, such as swabs or ice, may provide additional information for at-risk facilities. Typically, a 250 mL sample is sufficient for routine testing. visible debris and no detectable disinfectant residual). non-potable) or condition (detectable disinfectant residual vs. The volume of water you collect may depend on the source type (potable vs. ![]() Sampling location recommendations are included in the device-specific modules and CDC Sampling Procedure and Potential Sampling Sites for Investigations.
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